Detection of Aneuploidy in Human Sperm
نویسندگان
چکیده
منابع مشابه
Detection of aneuploidy in human sperm.
Adequate methods for monitoring any type of gametic mutation directly in man are virtually nonexistent. A method is presented by which one can monitor Y chromosomal nondisjunction directly in the male gamete by quantifying the number of spermatozoa with two fluorescent bodies (YFF) in 1000 sperm counted. Dried semen slides are stained with quinacrine dihydrochloride and examined under a fluores...
متن کاملImpact of DNA Damage on the Frequency of Sperm Chromosomal Aneuploidy in Normal and Subfertile Men
Background: Various frequencies of sperm aneuploidy are reported in sperms of subfertile patients compared to normal individuals. Moreover, sperm DNA damage is shown to be associated with male infertility. In this study, the rate of DNA damage and frequencies of aneuploidy in sperms of subfertile patients was investigated. Methods: Semen samples were obtained from healthy normal and subfertile ...
متن کاملAneuploidy Assessment of X, Y, 18 Chromosomes in Sperm of Oligoteratospermia Using FISH Technique
Introduction: Not being to get pregnant, after one year of unprotected sex is called infertility. In the post, infertility was mainly a female problem, but the role of male factors in infertility has denotation, although a greater percentage of this infertility is related to the deficiencies of semen. Methods: This case-control study was performed on sperm samples from 30 infertile oligoterato...
متن کاملSperm Aneuploidy and Dna Integrity: a Review
Male factors leading to infertility account for at least half of all cases of infertility worldwide. The purpose of this review is to highlight the importance of sperm DNA integrity. A systematic literature search was performed up to January 2015 in order to determine the impact of sperm DNA integrity and of the techniques used to determine it. Only articles presenting sperm aneuploidy together...
متن کاملDevelopment of a non-human primate sperm aneuploidy assay tested in the rhesus macaque (Macaca mulatta).
Numerical chromosome aberrations in germ cells are important factors contributing to abnormal reproductive outcomes. Fluorescence in situ hybridization onto spermatozoa (sperm-FISH) has allowed the study of the influence of a wide range of biological factors and chemical exposure on aneuploidy incidences in human sperm as well as in mouse and rat animal models. The assay presented here extends ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Environmental Health Perspectives
سال: 1979
ISSN: 0091-6765
DOI: 10.2307/3429138